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CHO Cell Serum-free Medium
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  • CHO Serum-free Cell Culture Medium

CHO Serum-free Cell Culture Medium


CHO cells are important hosts for antibody and recombinant protein production. Based on the production process characteristics of the mAb & recombinant protein industry, Duoning cell culture medium product development team focuses on the research of the culture medium formula mechanism, CHO cell metabolism mechanism and culture medium stability, and through the analysis by CHO cell metabolism model and rational experiment design, successfully designed and developed Media C series & DN feed series media products. Duoning CHO medium can meet the growth, metabolism and expression demand for di?erent types of CHO cells (CHOK1, CHOS, DG44, etc.). Media C series & DN feed series balance both high performance and good quality. Together with the upstream and downstream biopharmaceutical process products from Duoning, we can provide customers with complete solutions for di?erent bioprocessing scenarios.

 

CHO Serum-free Cell Culture Medium

 CHO Cell Culture Serum-Free Medium

 

CHO Serum-free Cell Culture MediumProduct Features

?   Completely chemically de?ned (CD) medium

?   Support high cell density suspension culture and achieve high productivity

?   Applicable to di?erent CHO cell types (CHOK1, CHOK1SV, CHOZN, CHOS, DG44, DUKB11, CHO-M, etc.) Provide convenient preparation methods for GMP production

?   Products have independent intellectual property rights

 

CHO Serum-free Cell Culture MediumProduct Benefits

?   Versatility: The Media C series combined with the DN feed series has good versatility and can well meet the platform needs of customers.

?   High productivity: Comparing to other products on market, the antibody titer can be signi?cantly improved and productivity of 12 g/L is achievable with fed-batch culture mode.

?   CQA control: Combined with Duoning quality regulator, we can e?ectively help customers to control the quality attributes of antibodies (glycoforms, charge variant and purity).

?   Convenience: It only requires a "three-step method" to complete the preparation, and no need to adjust the pH and osmotic pressure during the process.

?   Profession: Provide full technical support services, and the application team has supported and served more than 100 customers.

 

Media C series basal medium

Recommend cells

Media C-01

CHOK1, CHOS

Media C-02

CHOK1, CHOS, DG44

Media C-03

CHOK1, CHOZN, CHO-M

Media C-04

CHOK1

Media C-05

CHOK1, DG44

Media C-06

CHOK1, CHOS, CHOK1SV

 

DN feed series feed medium

Recommend cells

DN feed 3 + DN feed B3

CHOK1, CHOS, DG44 etc.

DN feed 4 + DN feed B4

CHOK1, CHOS, DG44 etc.

 

CHO Serum-free Cell Culture MediumProduct Performance Data

 

Case study 1:

Media C-01+DN feed 3/DN feed 4 combination

Recommended cell: CHOK1 (ATCC)

CHO Serum-free Cell Culture Medium

 

Case study 2:

Media C-03+DN feed 3/DN feed 4 combination

Recommended cell: CHOZN

 

CHO Serum-free Cell Culture Medium

 

Case study 3:

Media C-04+DN feed 3/DN feed 4 combination

Recommended cell: CHOK1 (ECACC)

 

CHO Serum-free Cell Culture Medium

 

Case study 4:

Media C-05+DN feed 3/DN feed 4 combination

Recommended cell: CHOK1 (ATCC)

 

CHO Serum-free Cell Culture Medium

 

Case study 5:

Media C-06+DN feed 3/DN feed 4 combination

Recommended cell: CHOK1SV

 

CHO Serum-free Cell Culture Medium

 

Case study 6:

Product quality regulation

 

Glycoform (galactosylation)


Titer

GO

GOF

Man5

G1

G1F

G2F

Original drug

/

0.76

85.04

0.41

0.38

8.65

0.62

Commercial medium

1 g/L

0.09

55.32

0.76

0.61

33.79

5.22

Duoning medium

2.5 g/L

0.81

86.12

0.39

0.41

9.11

0.52

 

CHO Serum-free Cell Culture MediumOrdering information

 

Product type

Product name

Specification

Product number

Product form

Basal Medium

Media C-01 medium

100 g

MP011-100g-02

Dry powder

1 kg

MP011-1kg-02

5 kg

MP011-5kg-02

10 kg

MP011-10kg-02

25 kg

MP011-25kg-02

Media C-03 medium

100 g

MP014-100g-02

1 kg

MP014-1kg-02

5 kg

MP014-5kg-02

10 kg

MP014-10kg-02

25 kg

MP014-25kg-02

Media C-04 medium

100 g

MP015-100g-02

1 kg

MP015-1kg-02

5 kg

MP015-5kg-02

10 kg

MP015-10kg-02

25 kg

MP015-25kg-02

Media C-05 medium

100 g

MP038-100g-02

1 kg

MP038-1kg-02

5 kg

MP038-5kg-02

10 kg

MP038-10kg-02

25 kg

MP038-25kg-02

Media C-06 medium

100 g

MP039-100g-02

1 kg

MP039-1kg-02

5 kg

MP039-5kg-02

10 kg

MP039-10kg-02

25 kg

MP039-25kg-02

Media C-03

500 ml

LB005-500ml-02

Liquid

1 L

LB005-1L-02

Media C-06

500 ml

LB008-500ml-02

1 L

LB008-1L-02

Feed Medium

DN feed 3 medium

100 g

FP015-100g-02

Dry powder

1 kg

FP015-1kg-02

5 kg

FP015-5kg-02

10 kg

FP015-10kg-02

25 kg

FP015-25kg-02

DN feed B3 medium

100 g

FP016-100g-02

1 kg

FP016-1kg-02

5 kg

FP016-5kg-02

10 kg

FP016-10kg-02

25 kg

FP016-25kg-02

DN feed 4 medium

100 g

FP021-100g-02

1 kg

FP021-1kg-02

5 kg

FP021-5kg-02

10 kg

FP021-10kg-02

25 kg

FP021-25kg-02

DN feed B4 medium

100 g

PF001-100g-02

1 kg

PF001-1kg-02

5 kg

PF001-5kg-02

10 kg

PF001-10kg-02

25 kg

PF001-25kg-02

 




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  • E-Mail: marketing@duoningbio.com
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