Heparin affinity resin can be applied for separation and purification of protein molecules that have specific binding with heparin molecules, including coagulation factors, antithrombin III, growth factors, interferon and lipase.
Heparin affinity resin uses super-macroporous polyacrylate hard gel as matrix and adopts unique chemical bonding technology from Bogen (A Duoning company) to immobilize heparin molecules, separating protein of interest through the specific binding between heparin and antithrombin III or protein molecules with similar structures.
Product features
? High protein-binding affinity
? High protein recovery
? Large pore size with low mass transfer resistance, suitable for purification of high viscosity samples
Case Study
Antithrombin III purification
Column size 1.0cm ID x 2.5 cm, CV=2ml
Buffer: Equilibration buffer A: 50mM Tris/HCI, pH7.2；Elution buffer B: 50mM Tris/HCI+IM NaCI, pH7.2
Flowrate: 1 ml/min
Feed: Pre-processed GMO goat milk (0.45μm membrane filtered)
1.Elution peak P1 of Bogen resin
2.Elution peak P2 of Bogen resin
3.Elution peak P1 of competitor’s resin
4.Elution peak P2 of competitor’s resin
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