GST-tag protein purification resin is specially designed for purification of GST (glutathione S-transferase) tag fusion protein, it can quickly capture protein of interest containing GST tag from the cell culture broth.

GST-tag protein purification resin is specially designed for purification of GST (glutathione S-transferase) tag fusion protein, it can quickly capture protein of interest containing GST tag from cell culture broth.

GST-tag protein purification resin uses polyacrylate hard gel or agarose soft gel as matrix, glutathione is conjugated by Bogen’s (A Duoning company) unique chemical bonding technology, the purification of targeted protein is achieved through the specific binding between glutathione and GST-tag fusion protein.

1、High protein affinity

2、High protein recovery

3、High binding capacity up to 15 mg/mL

GST fusion protein purification

Column size 1.0cm ID x 2.5 cm, CV=2ml

Buffer：Equilibration buffer A: 50mM Tris/HCI+0.15M NaCI，pH 8.0

Elution buffer B: 50mM Tris/HCI+10 mM GSH，pH 8.0

Flowrate: 1 ml/min

Feed: GST fusion protein culture broth (0.45μm filtered)

1.      Flow-through peak P0 of competitor’s resin

2.      Elution peak P1 of competitor’s resin

3.      Elution peak P1 of Bogen resin

4.      Flow-through peak P0 of Bogen resin