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Affinity Chromatography Resin
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  • His-tag protein purification resin

His-tag protein purification resin

His-tag protein purification resin is specially designed for purification of His-tag fusion proteins, it can capture His-tag fusion proteins from cell lysis/culture media. Depending on expression systems, you can choose products with different conjugated ligand types: IDA, NTA and Plus, among which, IDA and NTA are suitable for purifying His-protein expressed in E. coli cells, and Plus is suitable for purifying extracellularly expressed products from insect and mammalian cells.

His-tag protein purification resin is specially designed for purification of His-tag fusion proteins, it can capture His-tag fusion proteins from cell lysis/culture media. Depending on expression systems, you can choose products with different conjugated ligand types: IDA, NTA and Plus, among which, IDA and NTA are suitable for purifying His-protein expressed in E. coli cells, and Plus is suitable for purifying extracellularly expressed products from insect and mammalian cells.

 

His-tag protein purification resin (GP-IDA) uses super-macroporous polyacrylate beads as matrix, iminodiacetic acid (IDA) as chelating ligand, and conjugates Ni2+. This product features low mass transfer resistance and is suitable for capturing His-protein from high viscosity feed, including supernatant of E. coli cell lysis.

 

His-tag protein purification resin (CP-NTA) uses macroporous polyacrylate beads as matrix and nitrilotriacetic acid (NTA) as chelating ligand, which is resistant to low concentration of reducing agent added in protein solution, including B-mercaptoethanol or dithiothreitol (DTT).

 

His-tag protein purification resin (HP-Plus) uses polyacrylate beads as matrix and adopts unique metal chelating ligand (Plus) from Bogen (A Duoning company), which can stably adsorb Ni2+ without falling off even in solutions containing EDTA; and it can be CIP with NaOH while no need to be re-conjugate Ni2+. This product can greatly save time of sample pretreatment and after-process treatment of resin. It is especially suitable for purification of His-tag proteins expressed and secreted eukaryotic cells, including insect and mammalian cells.


Product features

Specifications

His-tag protein purification resin

GP-IDA

His-tag protein purification resin

GP-NTA

His-tag protein purification resin

HP-Plus

Matrix

Super macroporous polyacrylate hard gel

Macroporous polyacrylate hard gel

Polyacrylate hard gel

Ligand

IDA

NTA

Plus

Average particle size

70 μm

70 μm

70 μm

Reducing agent resistance

NO

β-Me≤100 mM

DTT≤5mM

EDTA≤50mM

Dynamic binding capacity

≥ 40 mg/ml

≥ 30 mg/ml

≥ 10 mg/ml

Max flow rate

1000 cm/hr

1000 cm/hr

1000 cm/hr

Max pressure

1 MPa

1 MPa

1 MPa

Storage

4-30 20% ethanol

4-30 20% ethanol

4-30 20% ethanol

Features

High protein capacity

Low metal ion leaching rate

Resistant to high concentration of reducing agents


For different purification requirements, Bogen also provides His-tag protein purification resins based on agarose soft gel matrix. Considering different His protein binding ability and transition metal ion selectivity, we also provide IMAC products without chelated metal ions, users can choose to chelate Cu2+, Zn2+, Co2+ and other transition metal ions.


Specifications

His-tag protein purification resin

NTA

His-tag protein purification resin

IMAC

Matrix

Agarose soft gel

Agarose soft gel

Ligand

NTA

IDA/NTA/Plus

Average   particle size

90 μm

90 μm

Max flow rate

500 cm/hr

500 cm/hr

Max pressure

0.3 MPa

0.3 MPa

Storage

4-8 20% ethanol

4-8 20% ethanol

Features

Low metal ion leaching rate

The type of metal ion can be freely selected


Case Study


Purifying His-fusion protein from supernatant of E.Coli lysis.

 

Column size 1.6cm ID x 2.5cm

BufferBuffer A: 20 mM PB+0.15M NaCI, pH6.0 (wash1)Buffer BBuffer A+10mM imidazole (wash2)Buffer CBuffer A+100mM imidazole (elute)

Flow rate5 ml/min

Detection UV wavelength280 nm

FeedCrude cell lysate 520 ml


His 標(biāo)簽蛋白純化填料





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