久久久精品欧美一区二区三区,国产欧美久久久精品影院,亚洲AV福利天堂一区二区三亚洲欧洲日韩国产AⅤ在线,亚洲成av人在线视,四虎国产精品永久地址99自拍三区一级国产片,亚洲男人的天堂久久无在线观看免费黄视频,亚洲一级特黄大片在线播放,国产成人精品区在线观看

Affinity Chromatography Resin
Home > Technology > Chromatography Resin > Affinity Chromatography Resin > His-tag protein purification resin
  • His-tag protein purification resin

His-tag protein purification resin

His-tag protein purification resin is specially designed for purification of His-tag fusion proteins, it can capture His-tag fusion proteins from cell lysis/culture media. Depending on expression systems, you can choose products with different conjugated ligand types: IDA, NTA and Plus, among which, IDA and NTA are suitable for purifying His-protein expressed in E. coli cells, and Plus is suitable for purifying extracellularly expressed products from insect and mammalian cells.

His-tag protein purification resin is specially designed for purification of His-tag fusion proteins, it can capture His-tag fusion proteins from cell lysis/culture media. Depending on expression systems, you can choose products with different conjugated ligand types: IDA, NTA and Plus, among which, IDA and NTA are suitable for purifying His-protein expressed in E. coli cells, and Plus is suitable for purifying extracellularly expressed products from insect and mammalian cells.

 

His-tag protein purification resin (GP-IDA) uses super-macroporous polyacrylate beads as matrix, iminodiacetic acid (IDA) as chelating ligand, and conjugates Ni2+. This product features low mass transfer resistance and is suitable for capturing His-protein from high viscosity feed, including supernatant of E. coli cell lysis.

 

His-tag protein purification resin (CP-NTA) uses macroporous polyacrylate beads as matrix and nitrilotriacetic acid (NTA) as chelating ligand, which is resistant to low concentration of reducing agent added in protein solution, including B-mercaptoethanol or dithiothreitol (DTT).

 

His-tag protein purification resin (HP-Plus) uses polyacrylate beads as matrix and adopts unique metal chelating ligand (Plus) from Bogen (A Duoning company), which can stably adsorb Ni2+ without falling off even in solutions containing EDTA; and it can be CIP with NaOH while no need to be re-conjugate Ni2+. This product can greatly save time of sample pretreatment and after-process treatment of resin. It is especially suitable for purification of His-tag proteins expressed and secreted eukaryotic cells, including insect and mammalian cells.


Product features

Specifications

His-tag protein purification resin

GP-IDA

His-tag protein purification resin

GP-NTA

His-tag protein purification resin

HP-Plus

Matrix

Super macroporous polyacrylate hard gel

Macroporous polyacrylate hard gel

Polyacrylate hard gel

Ligand

IDA

NTA

Plus

Average particle size

70 μm

70 μm

70 μm

Reducing agent resistance

NO

β-Me≤100 mM

DTT≤5mM

EDTA≤50mM

Dynamic binding capacity

≥ 40 mg/ml

≥ 30 mg/ml

≥ 10 mg/ml

Max flow rate

1000 cm/hr

1000 cm/hr

1000 cm/hr

Max pressure

1 MPa

1 MPa

1 MPa

Storage

4-30 20% ethanol

4-30 20% ethanol

4-30 20% ethanol

Features

High protein capacity

Low metal ion leaching rate

Resistant to high concentration of reducing agents


For different purification requirements, Bogen also provides His-tag protein purification resins based on agarose soft gel matrix. Considering different His protein binding ability and transition metal ion selectivity, we also provide IMAC products without chelated metal ions, users can choose to chelate Cu2+, Zn2+, Co2+ and other transition metal ions.


Specifications

His-tag protein purification resin

NTA

His-tag protein purification resin

IMAC

Matrix

Agarose soft gel

Agarose soft gel

Ligand

NTA

IDA/NTA/Plus

Average   particle size

90 μm

90 μm

Max flow rate

500 cm/hr

500 cm/hr

Max pressure

0.3 MPa

0.3 MPa

Storage

4-8 20% ethanol

4-8 20% ethanol

Features

Low metal ion leaching rate

The type of metal ion can be freely selected


Case Study


Purifying His-fusion protein from supernatant of E.Coli lysis.

 

Column size 1.6cm ID x 2.5cm

BufferBuffer A: 20 mM PB+0.15M NaCI, pH6.0 (wash1)Buffer BBuffer A+10mM imidazole (wash2)Buffer CBuffer A+100mM imidazole (elute)

Flow rate5 ml/min

Detection UV wavelength280 nm

FeedCrude cell lysate 520 ml


His 標(biāo)簽蛋白純化填料





Contact Us
  • Tel.: +86 021 6434 0155
  • E-Mail: marketing@duoningbio.com
  • Address: Building 30, No. 1525 Minqiang Road, Songjiang District, Shanghai
Message

Copyright ? Shanghai Duoning Biotechnology Co., Ltd. All Rights Reserved Sitemap | Technical Support: Reanod

WeChat
WeChat

Message-

Duoning Biotechnology

+86 021 6434 0155

中文字幕理伦午夜福利片| 久久久久噜噜噜亚洲熟女综合| 华人色视频在线观看| 亚洲日产乱码一二三区别| 亚洲AV无码一区二区三区观看| 一级做a爰片久久毛片看看| 无遮挡又黄又刺激的视频网站| 国产国语毛片在线看国产| 女人被狂躁C到高潮视频| 国产精品乱码高清在线观看| 欧美黑人肉体狂欢交换大派对| 动漫精品啪啪一区二区三区| 国产大神高清视频在线观看| 日韩人妻无码肉v视频| 国产在线精品一区二区不卡麻豆 | 日本免码va在线看免费最| 一边摸一边抽搐一进一出视频| 和邻居少妇愉情中文字幕| 日韩欧美一区二区三区在线播放| 777久久精品一区二区三区无码| 玖玖成人无码大片一区二区| 欧美A级毛欧美1级a大片免费播放| 国产在线观看无码免费视频| 亚洲精品免费视频观看视频| 成全视频免费观看在线下载| 日本伊人精品一区二区三区 | 久久久久久国产一级AV片| 国产精品色婷婷亚洲综合看片| 久久99国产综合精品尤物| 欧美大片欧美激情性色a∨在线| 日韩在线中文字幕国| 巨胸美女露双奶头无遮挡一丝网站 | 播放一区乱黄久久久精品亚洲中文字| 亚洲AV无码乱观看明星换脸VA| 99最新免费精品视频| 国产精品99久久久久久人| 日韩美女午夜精品视频| 迈开腿让我看一下小鸡蛋视频| 国产综合久久亚洲综合| 国产精品 1080P 在线精品视频一区二区三四| 国产丝袜拍偷超清在线|