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Cell Culture Flasks
Home > Technology > Laboratory Equipment & Comsumable > Cell Culture Flasks > Cell Culture Flask & Aseptic Transfer Cap

Cell Culture Flask & Aseptic Transfer Cap

 

Duoning cell culture flasks are manufactured with excellent and rigorous process, ensures the smooth body of the flasks, and reduces the risk of foaming during culture.

 

Duoning cell culture flasks have multiple uses, including cultivate mammalian cells (CHO cells, HEK 293 cells) for recombinant protein production. Excellent product design can greatly improve the oxygen transfer efficiency inside the flask itself , thereby providing better growth environment for cells.

 

Duoning cell culture flasks can be used with other cell culture equipment and consumables produced by Duoning, including carbon dioxide incubators with shakers, bioreactors.

 

Cell Culture FlaskProducts Benefits

 

?   Meet regulations of USP6

?   Blow-injection molding, avoiding surface scars

?   Excellent chemical resistance, can withstand a variety of chemicals

?   Good transparency, clear scale, easy to observe

?   Flask neck sizes conforms to the international SBS standard, suitable for quick connection of bioreactors

 

Cell Culture FlaskFeatures Of 2.8 L & 5 L Cell Culture Flasks

 

?   Equipped with a 0.2 μm air-permeable cap, which can better mix the cells and the culture media, increase the air permeability, and enable the cells to grow to higher density.

?   The handle design of the 5 L culture flask is easy to pick up, avoiding the risk of contamination caused by the customer's hand being too close to the flask neck during use.

?   The bottom of the 2.8 L culture bottle is optimized, which saves space, greatly improves the utilization rate of the incubator, and reduces customer R&D and production costs.

?   Duoning cell culture flask adopts extrusion blowing technology, the flask body is smooth, which reduces the risk of cell adhesion and media foaming.

?   Duoning cell culture flasks are sterilized by cobalt 60 radiation, and the aseptic independent packaging is convenient for use.

 

                                              Cell Culture Flask 

 

Aseptic transfer cap

 

Duoning aseptic transfer caps are used for aseptic transfer during the expansion of suspension cells, effectively reducing the risk of contamination. The aseptic transfer cap consists of two ports: The vent port is connected to a 0.2 um air filter; The inner end of the liquid delivery port is connected to a chemically resistant hard tube immersed in the bottom part of the flask, and the outer side is connected to a 1/8”-1/4” thermoplastic tube.

Cell Culture Flask

 

Cell Culture FlaskProduct benefits


?   Compatible with Duoning 2.8 L & 5 L culture flasks;

?   Double-layer independent aseptic packaging;

?   Disposable, ready-to-use.

 

Cell Culture FlaskOrdering information


Cell Culture Flask

Part No.

Volume

Type

Height

Neck Diameter

Bottom Diameter

Sterilization

Pcs/Pack

Pcs/Box

DNFL02010125

125 mL

PETG

113.5 mm

34 mm

66 mm

Yes

1

24

DNFL02010250

250 mL

138.2 mm

34 mm

83 mm

12

DNFL02010500

500 mL

177.5 mm

39 mm

101 mm

12

DNFL02011000

1,000 mL

211.5 mm

39 mm

127 mm

6

DNFL02032800

2.8 L

K-Resin

243.3 mm

96.2 mm

164 mm

6

03-5000

5 L, 4 pcs

253.2 mm

96.2 mm

228 mm

4

DNFL02020125

125 mL

PC

113.5 mm

34 mm

66 mm

24

DNFL02020250

250 mL

138.2 mm

34 mm

83 mm

12

DNFL02020500

500 mL

177.5 mm

39 mm

101 mm

12

DNFL02021000

1,000 mL

211.5 mm

39 mm

127 mm

6

 

Aseptic Transfer Cap

Part No.

Sterilization

Pcs/pack

Pcs/box

DNTC020106P

Yes

1

10

 

Culture Parameter Recommendation

Cell type

Size

Recommended max capacity (mL)

Max speed (rpm)

Shaker track specification (mm)

CHO-S

125 mL

30

130

25

250 mL

60

130

25

500 mL

150

130

25

1,000 mL

350

130

25

2,800 mL

800

90

25

5,000 mL

2,500

90

25

HEK

125 mL

30

130

15

SF9

125 mL

30

130

15

500 mL

200

150

31.75

1,000 mL

300

120-150

31.75

2,800 mL

1,000

100-150

31.75

5,000 mL

3,500

90-100

31.75

 

Cell Culture FlaskPerformance Data

CHOK1 cell growth results after 10x passages

 

Cell Culture Flask

 

Volume

Optimal working volume

Effective volume

Optimal speed

Suggested speed range

125 mL

25 – 30 mL

8 - 35 mL

110

90 - 110

250 mL

60 mL

36 – 100 mL

500 mL

100 mL

50 – 200 mL

1,000 mL

200 mL

101 – 400 mL

2.8 L

1.4 L

0.9 – 1.6 L

5 L

2 L

1.7 – 3.2 L

90

Note:Duoning culture flask specification only

 

 


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Contact Us
  • Tel.: +86 021 6434 0155
  • E-Mail: marketing@duoningbio.com
  • Address: Building 30, No. 1525 Minqiang Road, Songjiang District, Shanghai
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