DN feed 1+DN feed B
DN feed 1 is animal-free, protein-free, chemical-defined (CD) high-performance feed media, can be used for fed-batch culture of therapeutic protein product-producing Chinese hamster ovary (CHO) cells, through R&D to commercial manufacturing. DN feed 1 needs to be combined use with DN feed B, added to culture simultaneously.
Case study
Bispecific antibody expression performance of CHOS cell with various cell culture media. DN feed 2+DN feed B2
DN feed 2 is animal-free, protein-free, chemical-defined (CD) high-performance feed media, can be used for fed-batch culture of therapeutic protein product-producing Chinese hamster ovary (CHO) cells, through R&D to commercial manufacturing. DN feed 2 needs to be combined use with DN feed B2, added to culture simultaneously.
Case study
Cell growth and product expression with DN feed 2 + DN feed B2 vs. other commercial feed media |
How to use
We recommend to start adding feed media during middle-late stage of cell exponential growth stages, usually on day 3 when cell density reaches 2.0~3.0×106 cells/mL, followed by add every other day with 3%±0.3%. Users can optimize the feed strategy based on the cell lines.
DNFeed Series can be used for cell lines using GS and dhfr screening systems, including CHO-K1, CHO-S, DG44, CHOZN and CHOK1SV.
Ordering information
Product Name | Product No. | Spec. | Form | Shelf Life |
DN feed 1 | CDF1-001 | 1L | Liquid | 6 months |
CDFP1-001 | 100g | Powder | 2 years | |
CDFP1-010 | 1kg | Powder | 2 years | |
CDFP1-050 | 5kg | Powder | 2 years | |
DN feed B | CDB1-001 | 300mL | Liquid | 6 months |
CDBP1-001 | 100g | Powder | 2 years | |
CDBP1-010 | 1kg | Powder | 2 years | |
DN feed 2 | FT002-1L | 1L | Liquid | 6 months |
FP002-100g | 100g | Powder | 2 years | |
FP002-1kg | 1kg | Powder | 2 years | |
FP002-5kg | 5kg | Powder | 2 years | |
DN feed B2 | FT003-300mL | 300mL | Liquid | 6 months |
FP003-100g | 100g | Powder | 2 years | |
FP003-1kg | 1kg | Powder | 2 years |
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