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Hydrophobic Interaction Chromatography Resin
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Hydrophobic interaction chromatography


Hydrophobic interaction chromatography (HIC) is not commonly used in protein bioprocesses, but for particular products, binding and separation rely on the hydrophobic interaction between resin and protein molecular. The hydrophobic interaction chromatography process features "high salt for binding" and “l(fā)ow salt for elution”, and usually use in series with affinity, ion exchange and gel f1Ltration chromatography steps. Hydrophobic interaction chromatography has high requirements for technologies of hydroph1Lic surface modification of resin.

Hydrophobic interaction chromatography

 

Bogen (A Duoning Company) uses high-strength hydroph1Lic polyacrylate polymer as matrix, and produces hydrophobic resins with uniform distribution, stable structure and strong controllab1Lity through industry-leading multi-scale micro-nano structure control technology; meanwh1Le, we adopt flexible hydroph1Lic modification technology, to further avoid non-specific absorption, ensuring high recovery rate. Bogen provides customized services of hydrophobic interaction resins with different ligand density, helping users to develop optimum purification process.

 

Product Series

?   HP series: use for purification of peptide and recombinant proteins

?   CP series: use for purification of recombinant proteins and antibodies

?   GP series: use for purification of antigens of vaccines and viruses

 

Average particle size: 40m, 70μm

 

Hydrophobic interaction chromatographyProduct Features

 

Series

Product Name

Average Particle Size

Pore Size

Max Pressure

Max Flowrate

Hydrophobic Group Density

HP

Phenyl hydrophobic interaction media

(low density)

40 μm

70 μm

30nm

1MPa

1500cm/h

15μmol/mL

Phenyl hydrophobic interaction media

(high density)

35μmol/mL

Butyl hydrophobic interaction resin

(low density)

20μmol/mL

Butyl hydrophobic interaction resin

(high density)

40μmol/mL

CP

Phenyl hydrophobic interaction resin

(low density)

40 μm

70 μm

100nm

1MPa

1500cm/h

15μmol/mL

Phenyl hydrophobic interaction resin

(high density)

35μmol/mL

Butyl hydrophobic interaction resin

(low density)

20μmol/mL

Butyl hydrophobic interaction resin

(high density)

40μmol/mL

GP

Phenyl hydrophobic interaction resin

(low density)

40 μm

70 μm

500-1000nm

1MPa

1500cm/h

15μmol/mL

Phenyl hydrophobic interaction media

(high   density)

35μmol/mL

Butyl hydrophobic interaction resin

(low density)

20μmol/mL

Butyl hydrophobic interaction resin

(high density)

40μmol/mL

 

Ligand Types

Ligand type is critical factor affecting the selectivity of hydrophobic chromatography resin. Commonly used hydrophobic ligands include: phenyl, octyl, butyl, butylsulfide, butylamine, polyethylene glycol, and polyaldehyde. When the density of hydrophobic ligand is high, for example, more benzene rings or longer hydrophobic chain, both hydrophobicity and protein binding of the resin w1Ll be stronger, but difficult to elute when too strong. The order of hydrophobicity of the ligand is: phenyl>octyl>butyl.

 

Ligand Density

The ligand density of hydrophobic interaction chromatography resins should be selected in an appropriate range: , protein molecules are prone to multi-site binding and cannot be eluted if the density is too high, and even causing protein structure changes or denaturation and inactivation; if the density is too low, the protein binding capacity w1Ll be low or even cannot bind to resin.

 

Case Study

 

Hydrophobic interaction chromatography

Chromogram plasmid purification by GPB-60 butyl hydrophobic interaction resin

 

Bogen GPB-60Butyl hydrophobic interaction resin can efficiently separate scDNA and ocDNA.

 

Hydrophobic interaction chromatographyOrdering Information

 

Hydrophobic Interaction Resin

HP Series

Product Name

Part No.

Specification

Product Name

Part No.

Specification

HPP-60 Phenyl hydrophobic interaction resin (low density)

BG41-1110-02

50mL

HPP-30 Phenyl hydrophobic interaction resin (low density)

BG41-1210-02

50mL

BG41-1110-04

500mL

BG41-1210-04

500mL

BG41-1110-05

1L

BG41-1210-05

1L

BG41-1110-07

10L

8G41-1210-07

10L

BG41-1110-08

20L

BG41-1210-08

20L

HPP-60 Phenyl hydrophobic interaction resin (high density)

BG41-1111-02

50mL

HPP-30 Phenyl hydrophobic interaction resin (high density)

BG41-1211-02

50mL

BG41-1111-04

500mL

8G41-1211-04

500mL

BG41-1111-05

1L

BG41-1211-05

1L

BG41-1111-07

10L

BG41-1211-07

10L

BG41-1111-08

20L

BG41-1211-08

20L

HPB-60 Butyl hydrophobic interaction resin (low density)

BG43-1110-02

50mL

HPB-30 Butyl hydrophobic interaction resin (low density)

BG43-1210-02

50mL

BG43-1110-04

500mL

BG43-1210-04

500mL

BG43-1110-05

1L

BG43-1210-05

1L

BG43-1110-07

10L

BG43-1210-07

10L

BG43-1110-08

20L

BG43-1210-08

20L

HPB-60 Butyl hydrophobic interaction resin (high density)

BG43-1111-02

50mL

HPB-30 Butyl hydrophobic interaction resin (high density)

BG43-1211-02

50mL

BG43-1111-04

500mL

BG43-1211-04

500mL

BG43-1111-05

1L

BG43-1211-05

1L

BG43-1111-07

10L

8G43-1211-07

10L

BG43-1111-08

20L

BG43-1211-08

20L

HPQ-60 Octyl hydrophobic interaction resin

BG42-1110-02

50mL

HPQ-30 Octyl hydrophobic interaction resin

BG42-1210-02

50mL

BG42-1110-04

500mL

BG42-1210-04

500mL

BG42-1110-05

1L

BG42-1210-05

1L

BG42-1110-07

I0L

BG42-1210-07

10L

BG42-1110-08

20L

BG42-1210-08

20L

 

CP Series

Product Name

Part No.

Specification

Product Name

Part No.

Specification

CPP-60 Phenyl hydrophobic interaction resin (low density)

BG41-2110-02

50mL

CPP-30 Phenyl hydrophobic interaction resin (low density)

BG41-2210-02

50mL

BG41-2110-04

500mL

BG41-2210-04

500mL

BG41-2110-05

1L

BG41-2210-05

1L

BG41-2110-07

10L

BG41-2210-07

10L

BG41-2110-08

20L

BG41-2210-08

20L

CPP-60 Phenyl hydrophobic interaction resin (high density)

BG41-2111-02

50mL

CPP-30 Phenyl hydrophobic interaction resin (high density)

BG41-2211-02

50mL

BG41-2111-04

500mL

BG41-2211-04

500mL

BG41-2111-05

1L

BG41-2211-05

1L

BG41-2111-07

10L

BG41-2211-07

10L

BG41-2111-08

20L

BG41-2211-08

20L

CPB-60 Butyl hydrophobic interaction resin (low density)

BG43-2110-02

50mL

CPB-30 Butyl hydrophobic interaction resin (low density)

BG43-2210-02

50mL

BG43-2110-04

500mL

BG43-2210-04

500mL

BG43-2110-05

1L

BG43-2210-05

1L

BG43-2110-07

10L

BG43-2210-07

10L

BG43-2110-08

20L

BG43-2210-08

20L

CPB-60 Butyl hydrophobic interaction resin (high density)

BG43-2111-02

50mL

CPB-30 Butyl hydrophobic interaction resin (high density)

BG43-2211-02

50mL

BG43-2111-04

500mL

BG43-2211-04

500mL

BG43-2111-05

1L

BG43-2211-05

1L

BG43-2111-07

10L

BG43-2211-07

10L

BG43-2111-08

20L

BG43-2211-08

20L

CPQ-60 Octyl hydrophobic interaction resin

BG42-2110-02

50mL

CPQ-30 Octyl hydrophobic interaction resin

BG42-2210-02

50mL

BG42-2110-04

500mL

BG42-2210-04

500mL

BG42-2110-05

1L

BG42-2210-05

1L

BG42-2110-07

10L

BG42-2210-07

10L

BG42-2110-08

20L

BG42-2210-08

20L

 

GP Series

Product Name

Part No.

Specification

Product Name

Part No.

Specification

GPP-60 Phenyl hydrophobic interaction resin (low density)

BG41-3110-02

50mL

GPP-30 Phenyl hydrophobic interaction resin (low density)

BG41-3210-02

50mL

BG41-3110-04

500mL

BG41-3210-04

500mL

BG41-3110-05

1L

BG41-3210-05

1L

BG41-3110-07

10L

BG41-3210-07

10L

BG41-3110-08

20L

BG41-3210-08

20L

GPP-60 Phenyl hydrophobic interaction resin (high density)

BG41-3111-02

50mL

GPP-30 Phenyl hydrophobic interaction resin (high density)

BG41-3211-02

50mL

BG41-3111-04

500mL

BG41-3211-04

500mL

BG41-3111-05

1L

BG41-3211-05

1L

BG41-3111-07

10L

BG41-3211-07

10L

BG41-3111-08

20L

BG41-3211-08

20L

GPB-60Butyl hydrophobic interaction resin (low density)

BG43-3110-02

50mL

GPB-30 Butyl hydrophobic interaction resin (low density)

BG43-3210-02

50mL

BG43-3110-04

500mL

BG43-3210-04

500mL

BG43-3110-05

1L

BG43-3210-05

1L

BG43-3110-07

10L

BG43-3210-07

10L

BG43-3110-08

20L

BG43-3210-08

20L

GPB-60 Butyl hydrophobic interaction resin (high density)

BG43-3111-02

50mL

GPB-30 Butyl hydrophobic interaction resin (high density)

BG43-3211-02

50mL

BG43-3111-04

500mL

BG43-3211-04

500mL

BG43-3111-05

1L

BG43-3211-05

1L

BG43-3111-07

10L

BG43-3211-07

10L

BG43-3111-08

20L

BG43-3211-08

20L

GPQ-60 Octyl hydrophobic interaction resin

BG42-3110-02

50mL

GPQ-30 Octyl hydrophobic interaction resin

BG42-3210-02

50mL

BG42-3110-04

500mL

BG42-3210-04

500mL

BG42-3110-05

1L

BG42-3210-05

1L

BG42-3110-07

10L

BG42-3210-07

10L

BG42-3110-08

20L

BG42-3210-08

20L

 

Hydrophobic interaction chromatography resin prepacked column

Product Name

Specification

Applicable MW

Max Pressure

Max Flowrate

Application/Feature

HPP-60 Phenyl HIC column (low density)

5mL
  10mL

≤200KD

1MPa

1000cm/h

Scale-up study for hydrophobic interaction chromatography process

HPP-60 Phenyl HIC column (high density)

5mL
  10mL

≤200KD

1MPa

1000cm/h

HPB-60 Butyl HIC column (low density)

5mL
  10mL

≤200KD

1MPa

1000cm/h

HPB-60 Butyl HIC column (high density)

5mL
  10mL

≤200KD

1MPa

1000cm/h

HPQ-60 Octyl HIC column

5mL
  10mL

≤200KD

1MP3

1000cm/h

CPP-60 Phenyl HIC column (low density)

5mL

10mL

 100KD-300KD

1MPa

1000cm/h

Scale-up study for hydrophobic interaction chromatography process of high molecular weight protein

CPP-60 Phenyl HIC column (high density)

5mL

10mL

100KD-300KD

1MPa

1000cm/h

CPB-60 Butyl HIC column (low density)

5mL

10mL

100KD-300KD

1MPa

1000cm/h

CPB-60 Butyl HIC column (high density)

5mL
  10mL

100KD-300KD

1MPa

1000cm/h

CPQ-60 Octyl HIC column

5mL
  10mL

100KD-300KD

1MPa

1000cm/h

GPP-60 Phenyl HIC column (low density)

5mL
  10mL

≥150KD

1MPa

1000cm/h

Scale-up study for hydrophobic interaction chromatography process of ultra-high   molecular weight protein, viruses, virus-like particles

GPP-60 Phenyl HIC column (high density)

5mL
  10mL

≥150KD

1MPa

1000cm/h

GPB-60 Butyl HIC column (low density)

5mL
  10mL

≥150KD

1MPa

1000cm/h

GPB-60 Butyl HIC column (high density)

5mL
  10mL

≥150KD

1MPa

1000cm/h

GPO-60 Octyl HIC column

5mL
  10mL

150KD

1MPa

1000cm/h

 

 


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