Ion exchange resin is one of the most commonly used protein purification methods, which can separate target protein from impurities based on the difference in the isoelectric point of the proteins.

The hard gel chromatography resin developed by Bogen (A Duoning company) uses high-strength hydrophilic polyacrylate beads as matrix, and unique flexible hydrophilic modification technology to further eliminate the non-specific binding caused by the strong hydrophobic micro-surface of chromatography resins based on hard gel, which increases the recovery of active protein, improves the robustness and repeatability of process.

Bogen provides wide range of particle sizes for different purification process stages: for capture or crude feed purification, we recommend chromatography resin products with average particle size of 70 μm or 120 μm, for high-viscosity feed, consider resins with particle size of 120 μm or 200 μm; for polishing step, we recommend resins with average particle size of 40 μm or 70 μm. And please contact Bogen for more supports, if you need other chromatography resin products with non-standard particle sizes.

Max pressure up to 10 bar

Liner flowrate up to 1000 cm/h

Loading capacity 60-120mg/ml (depends on application)

DEAE、Q、CM、S

HP series：use for purification of peptide and recombinant proteins

CP series：use for purification of recombinant proteins and antibodies

GP series：use for purification of antigens of vaccine and virus-based products

Standard particle size：40μm、70μm

Bogen uses a series of patented technologies to optimize the particle size, pore structure and micro-surface physical and chemical properties of polymer hard gel, achieving higher basic resolution for resin itself. Comparing to agarose resins, it provides higher resolution under same process conditions.

Ion exchange resin: A Bogen HPS-60；B capto S；C capto S ImpAct

Column size：1.6cm ID x 2.5cm

Buffer：Buffer A: 20mM PBS, pH6.5；Buffer B：Buffer A + 0.5M NaCI

Flowrate: 2 ml/min

Gradient：A to B in 35min

Detection UV wavelength: 280nm

Loading Volume: 5ml

Feed: 1.Cytochrome C (1mg/ml)；2. Lysozyme (1mg/ml)

Besides high resolution, the chromatography resin from Bogen provides ideal pressure-flowrate curve, which helps to maintain low back pressure during scale-up. Under increased flowrate or column height, the pressure change is relatively smooth, and much lower than agarose-based resin, which is desired for scale-up production processes.

Pressure vs. flowrate for HPS strong cation exchange resin

Bogen uses multi-scale micro-nano structure control technology to produce chromatography resin with high specific surface area porous structure. We designed resins with optimum pore size and arm space through computer simulation technology and analyzing hydraulic size of different types of biomacromolecules, achieving high dynamic binding capacity and adsorption and desorption in fast mass transfer.

Column size：1 ml Prepacked column

Buffer：Buffer A: 20 mM Tris/HC1, pH 8.0；Buffer B: 20 mM Tris/HC1+1M NaCI, pH 8.0

Flow rate: 1.0 ml/min

Feed: 2 mg BSA/ml

Detection UV wavelength：280 nm

Vaccine/viruse purification is featured application of super macroporous ion-exchange resin developed by Bogen. Purifying vaccine antigen/virus vector using GPQ strong anion exchange resin or GPS strong cation exchange resin provides advantages of high loading (1-30 CV), high purity and high yield.

Application

Virus vaccine purification

Virus-like particles (VLP) purification

Virus vector purification, including LV、AV、 AAV

Purification of vaccine antigen

Ion exchange resin: GPQ-60

Column size1.6cmID x 15cm

Buffer：Buffer A: 20mM Tris/HCI pH 8.0；Buffer B: 20mM Tris/HC1 + 0.5M NaCI

Flowrate：5 ml/min

Feed：Vaccine antigen