MixQ virus purification resin is mixed mode resin developed by Bogen (A Duoning Company) for isolation and purification of virus-based products. This product features a core-shell structure. The shell is hydrophilic polymer with defined pore size, due to large size, the virus particles flow through under exclusion effect, while the small impurities pass through the shell and enter the core. The core is polymethacrylate with rigid structure, together with n-octylamine as ligand, which absorbs impurities through ion exchange/hydrophobic interaction, thus separating viruses and impurities.
We provide two types of MixQ virus purification resins: MixQ700 (>MW 700,000) and MixQ400 (>MW 400,000), based on the molecular weight of size exclusion。
Product features：
Mode of virus flow-through, impurity adsorb
Impurity proteins can also be adsorbed at high salt concentrations without buffer exchange
The composition of the virus flow-through solution is same as feed solution, maintaining virus activity
Product Features
Specifications | ||||
Products name | MixQ700S virus purification resin | MixQ700L virus purification resin | MixQ400S virus purification resin | MixQ400L virus purification resin |
Average particle size | 70 μm | 120 μm | 70 μm | 120 μm |
Size exclusion MW | ≥700 kD | ≥ 400 kD | ||
Max pressure | 1 MPa | 1 MPa | ||
Matrix | Polyacrylate hard gel | Polyacrylate hard gel | ||
Function group | N-octylamine | N-octylamine | ||
Dynamic binding capacity | ≥ 10 mg/ml | ≥ 10 mg/ml | ||
pH stability | Resistant to 0.5 M NaOH + 30% IPA clean | Resistant to 0.5 M NaOH + 30% IPA clean | ||
Storage | 4-30 ℃（20% ethanol）or 5-10 mM NaOH | 4-30 ℃（20% ethanol）or 5-10 mM NaOH | ||
Case Study
Purification of adenovirus vector
Resin：MixQ700L virus purification resin
Volume：1ml
Buffer：Buffer A：20 mM Tris-HCI + 50 mM NaCI，pH 8.0；Buffer B：0.5 M NaOH + 30% isopropyl alcohol
Flow rate：1.0 ml/min
Feed：Antigen of vaccine
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